disc1 shrna  (Santa Cruz Biotechnology)

Journal: Neural Plasticity

Article Title: Neurexin-Neuroligin Synaptic Complex Regulates Schizophrenia-Related DISC1/Kal-7/Rac1 “Signalosome”

doi: 10.1155/2015/167308

Figure Lengend Snippet: List of primers used in the study.

Article Snippet: An expression vector that encodes mouse FLAG-myc-tagged DISC1 was purchased from Origene (Rockville, MD, USA).

Techniques:

Journal: Neural Plasticity

Article Title: Neurexin-Neuroligin Synaptic Complex Regulates Schizophrenia-Related DISC1/Kal-7/Rac1 “Signalosome”

doi: 10.1155/2015/167308

Figure Lengend Snippet: NX1 β decreases the mRNA level of DISC1. Cortical neurons were grown for 8 days and subsequently treated with different concentrations of recombinant NX1 β for (a) 24 h and (b) 48 h. Total RNA was isolated, and mRNA levels were assessed using RT-PCR. The results from n = 3 experiments are expressed as a percentage ± SEM. ∗ P < 0.05, compared with the untreated control set at 100%.

Article Snippet: An expression vector that encodes mouse FLAG-myc-tagged DISC1 was purchased from Origene (Rockville, MD, USA).

Techniques: Recombinant, Isolation, Reverse Transcription Polymerase Chain Reaction

Journal: Neural Plasticity

Article Title: Neurexin-Neuroligin Synaptic Complex Regulates Schizophrenia-Related DISC1/Kal-7/Rac1 “Signalosome”

doi: 10.1155/2015/167308

Figure Lengend Snippet: Neuroligin 1 interacts with kalirin 7. (a) 293T cells were transfected with HA-tagged NL1-AB or myc-tagged Kal-7. Cell lysates were mixed and subsequently immunoprecipitated with anti-HA, anti-myc, or anti-Kal antibody, and immunoblots were probed with the indicated antibodies. Mouse IgG was used as a control. (b) HEK293 cells were transfected with HA-tagged NL1-AB or myc-tagged Kal-7 or FLAG-tagged DISC1. Cell lysates were mixed as indicated and subsequently immunoprecipitated. Immunoblots were probed with the indicated antibodies. Representative immunoblots of n = 3 experiments are shown.

Article Snippet: An expression vector that encodes mouse FLAG-myc-tagged DISC1 was purchased from Origene (Rockville, MD, USA).

Techniques: Transfection, Immunoprecipitation, Western Blot

Journal: Neural Plasticity

Article Title: Neurexin-Neuroligin Synaptic Complex Regulates Schizophrenia-Related DISC1/Kal-7/Rac1 “Signalosome”

doi: 10.1155/2015/167308

Figure Lengend Snippet: Schematic illustration of possible consequences of the NR1:NL1 interaction with the DISC1/Kal-7/Rac1 signalosome. At the baseline condition, NL1 forms homodimer interacting with NMDA receptor while DISC1 binds Kal-7, blocking the access of Kal-7 to Rac1. Upon synaptic activity and activation of NMDA receptors, NX1 β is cleaved from the presynaptic membrane leading to partial cleavage of NL1 from the postsynaptic membrane followed by the release of NL1 from NMDA receptor and DISC1 from Kal-7. After the shedding, NL1 is no longer a dimer which probably changes the mobility of N-terminally truncated NL1, and it can diffuse allowing NL1 to bind Kal-7 and in turn lead to the activation of Rac1. These events eventually allow NL1 to bind Kal-7 and in turn lead to the activation of Rac1. Additionally, binding of ecto-NX1 β t ο NL1 results in NR1 subunit phosphorylation at S896 and S897 and phosphorylation of ERK and Akt.

Article Snippet: An expression vector that encodes mouse FLAG-myc-tagged DISC1 was purchased from Origene (Rockville, MD, USA).

Techniques: Blocking Assay, Activity Assay, Activation Assay, Binding Assay